The ladder consists of nine bands in even increments 0. By the use of these products you accept the terms and conditions of all applicable limited use label licenses. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Tridye 1 kb plus dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
This product is available in quickload purple format enjoy the convenience of a readytoload format. The 1k and 3k bands have increased intensity to serve as. Limited product warranty life technologies corporation andor it affiliates warrant their products as set forth in the life technologies general terms and conditions. The following reagents are supplied with this product. Sigmas directload 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. The 1650bp band contains approximately 8% of the mass applied to the. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. The invitrogen trackit 1kb plus dna ladder is designed for sizing and quantification of double stranded dna on 0.
The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. For the convenience, the amounts of the 500, 1kbp and 5kbp fragments are adjusted to show greater intensities. How can i prepare working solution with 1 kb dna ladder supplied by promega. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restrict. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis.
The trackit 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments ranging in size from 100 bp to 15,000 bp. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Quickload 1 kb plus dna ladder new england biolabs. The product is supplied in a solution of 10 mm trishcl, ph 8. Pdf new bruceladder multiplex pcr assay for the biovar. Quickload 1 kb plus dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Directload 1 kb dna ladder readytouse marker for dna. There have been no changes to product formulation or specifications.
In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. View notes 1 kb plus dna ladder from biology 201 at new york university. How can i prepare working solution with 1 kb dna ladder. For na electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. This marker can be used with either agarose or polyacrylamide gels. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to.
Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Find and purchase 1 kb plus dna ladder products at invitrogen life science technologies. Generuler 1 kb plus dna ladder thermo fisher scientific. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Not intended for any animal or human therapeutic or diagnostic use. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. Generuler 1 kb plus dna ladder catalog number sm31, sm32 pub. Life technologies corporation andor its affiliates warrant their products as. Invitrogen 1kb dna extension ladder 100ug nucleic acid. How to dilute dna ladders andersen lab, 2016 invitrogen 1. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. This ladder was not designed for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size.
Use the 1 kb plus dna ladder is to size linear doublestranded dna fragments from 100 bp to 12 kb. How to dilute dna ladders andersen lab, 2016 we currently have two different kinds of ladder in the lab. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and. The dna includes fragments ranging from 25010,000 base pairs.
Trackit 1kb plus dna ladder thermo fisher scientific. The 1kb plus dna ladder is suitable for sizing double stranded dna. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Download fulltext pdf new bruceladder multiplex pcr assay for the biovar typing of article pdf available in veterinary microbiology 15412. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Formation of diffused bands of small dna fragments is a feature of agarose gel electrophoresis. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer.
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